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aec chromogen kit romulin aec chromogen kit  (Biocare Medical)
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Biocare Medical aec chromogen kit romulin aec chromogen kit
Expression of IL-19 and IL-19 receptors in lung tissues. (A) C57BL/6 mice were intranasally (i.n.) challenged with Der p extract (25 μg/mouse/day) for 10 days to induce asthma. (B) Dynamic serum levels of IL-19, IL-13, and immunoglobulin E (IgE) in Der p -sensitized mice ( n = 5). * P < 0.05, ** P < 0.01 compared with day 0 control by one-way non-parametric ANOVA. (C) Lung tissues were collected on day 13 and paraffin-sectioned for <t>IHC</t> <t>staining</t> for IL-19. Detection of the expression of surfactant protein C (SPC) in adjacent slide was used as marker for type II alveolar cells. (D) IHC staining for the expression of IL-20R1 and IL-20R2 with specific antibodies. Nuclei were counterstained with hematoxylin. Bars represented 50 μm. AM, alveolar macrophages; ATII, type II alveolar cells; <t>AEC,</t> airway epithelial cells.
Aec Chromogen Kit Romulin Aec Chromogen Kit, supplied by Biocare Medical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Expression of IL-19 and IL-19 receptors in lung tissues. (A) C57BL/6 mice were intranasally (i.n.) challenged with Der p extract (25 μg/mouse/day) for 10 days to induce asthma. (B) Dynamic serum levels of IL-19, IL-13, and immunoglobulin E (IgE) in Der p -sensitized mice ( n = 5). * P < 0.05, ** P < 0.01 compared with day 0 control by one-way non-parametric ANOVA. (C) Lung tissues were collected on day 13 and paraffin-sectioned for IHC staining for IL-19. Detection of the expression of surfactant protein C (SPC) in adjacent slide was used as marker for type II alveolar cells. (D) IHC staining for the expression of IL-20R1 and IL-20R2 with specific antibodies. Nuclei were counterstained with hematoxylin. Bars represented 50 μm. AM, alveolar macrophages; ATII, type II alveolar cells; AEC, airway epithelial cells.

Journal: Frontiers in Immunology

Article Title: Blocking IL-19 Signaling Ameliorates Allergen-Induced Airway Inflammation

doi: 10.3389/fimmu.2019.00968

Figure Lengend Snippet: Expression of IL-19 and IL-19 receptors in lung tissues. (A) C57BL/6 mice were intranasally (i.n.) challenged with Der p extract (25 μg/mouse/day) for 10 days to induce asthma. (B) Dynamic serum levels of IL-19, IL-13, and immunoglobulin E (IgE) in Der p -sensitized mice ( n = 5). * P < 0.05, ** P < 0.01 compared with day 0 control by one-way non-parametric ANOVA. (C) Lung tissues were collected on day 13 and paraffin-sectioned for IHC staining for IL-19. Detection of the expression of surfactant protein C (SPC) in adjacent slide was used as marker for type II alveolar cells. (D) IHC staining for the expression of IL-20R1 and IL-20R2 with specific antibodies. Nuclei were counterstained with hematoxylin. Bars represented 50 μm. AM, alveolar macrophages; ATII, type II alveolar cells; AEC, airway epithelial cells.

Article Snippet: The immune-reactivity of positive staining was developed by using the AEC chromogen kit (Romulin AEC Chromogen Kit; Biocare Medical, Walnut Creek, CA) and counterstained with Mayer's hematoxylin (J. T. Baker, Phillipsburg, NJ).

Techniques: Expressing, Control, Immunohistochemistry, Marker